iNOS Rabbit Polyclonal Antibody
cat.: ER1706-89
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Chicken |
| Applications: | IF-Cell, FC, WB, IHC-Fr |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 131 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human iNOS aa 1,104-1,153 / 1,153. |
| Positive control: | RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate, RAW264.7 whole cell lysate, HeLa cell lysate, A549 cell lysate, A549, LOVO. |
| Subcellular location: | Cytoskeleton, Nucleus, Cytosol. |
| Recommended Dilutions:
WB IF-Cell FC IHC-Fr |
1:1,000 1:50-1:200 1:50-1:100 1:100 |
| Uniprot #: | SwissProt: P35228 Human | P29477 Mouse |
| Alternative names: | HEP-NOS Hepatocyte NOS HEPNOS inducible Inducible nitric oxide synthase Inducible NO synthase Inducible NOS iNOS MAC NOS Macrophage NOS Nitric oxide synthase 2 inducible Nitric oxide synthase 2 inducible macrophage nitric oxide synthase 2A (inducible, hepatocytes) Nitric oxide synthase Nitric oxide synthase inducible nitric oxide synthase, macrophage NOS 2 NOS Nos II NOS type II nos2 NOS2_HUMAN NOS2A NOS2A, Inducible, Hepatocyte Peptidyl-cysteine S-nitrosylase NOS2 |
Images
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Fig1:
Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (ER1706-89) at 1/1,000 dilution. Lane 1: RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate (20 µg/Lane) Lane 2: RAW264.7 whole cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (30 µg/Lane) Lane 4: A549 cell lysate (30 µg/Lane) Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-89) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
ICC staining iNOS in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining iNOS in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Flow cytometric analysis of LOVO cells with iNOS antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
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Fig5:
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling iNOS with Rabbit anti-iNOS antibody (ER1706-89). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ER1706-89, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig6:
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling iNOS with Rabbit anti-iNOS antibody (ER1706-89). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ER1706-89, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.