iNOS Rabbit Polyclonal Antibody
cat.: ER1706-89
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: ICC, IHC-P, FC, WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 131 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human iNOS aa 1008-1152.
Positive control: A549, LOVO, human tonsil tissue, human spleen tissue, mouse small intestine tissue.
Subcellular location: Cytoskeleton. Nucleus. Cytosol.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P35228 Human | P29477 Mouse
Alternative names: HEP-NOS Hepatocyte NOS HEPNOS inducible Inducible nitric oxide synthase Inducible NO synthase Inducible NOS iNOS MAC NOS Macrophage NOS Nitric oxide synthase 2 inducible Nitric oxide synthase 2 inducible macrophage nitric oxide synthase 2A (inducible, hepatocytes) Nitric oxide synthase Nitric oxide synthase inducible nitric oxide synthase, macrophage NOS 2 NOS Nos II NOS type II nos2 NOS2_HUMAN NOS2A NOS2A, Inducible, Hepatocyte Peptidyl-cysteine S-nitrosylase NOS2
Images
ER1706-89_1.jpg Fig1: ICC staining iNOS in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-89_2.jpg Fig2: ICC staining iNOS in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-89_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-iNOS antibody. Counter stained with hematoxylin.
ER1706-89_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-iNOS antibody. Counter stained with hematoxylin.
ER1706-89_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-iNOS antibody. Counter stained with hematoxylin.
ER1706-89_6.jpg Fig6: Flow cytometric analysis of LOVO cells with iNOS antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.