Smad4 Rabbit Polyclonal Antibody
cat.: ER1706-90
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Smad4 aa 500-542. |
| Positive control: | A549, Hela, SH-SY5Y, rat large intestine tissue, human placenta tissue, mouse small intestine tissue, HUVEC. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q13485 Human | P97471 Mouse | O70437 Rat |
| Alternative names: | (Small) Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma 4 Deleted in Pancreatic Carcinoma Deleted in pancreatic carcinoma locus 4 Deletion target in pancreatic carcinoma 4 DPC 4 DPC4 hSMAD4 JIP MAD homolog 4 MAD mothers against decapentaplegic Drosophila homolog 4 MAD mothers against decapentaplegic homolog 4 MADH 4 MADH4 Med Medea Mothers against decapentaplegic homolog 4 Mothers against decapentaplegic, Drosophila, homolog of, 4 Mothers against DPP homolog 4 MYHRS OTTHUMP00000163548 SMA- and MAD-related protein 4 SMAD 4 SMAD family member 4 SMAD mothers against DPP homolog 4 SMAD4 SMAD4_HUMAN |
Images
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Fig1:
ICC staining Smad4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining Smad4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Smad4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Smad4 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Smad4 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Smad4 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of HUVEC cells with Smad4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.