SHP-2 Rabbit Polyclonal Antibody
cat.: ER1706-93
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SHP-2 aa 359-575. |
| Positive control: | A431, LOVO, MCF-7, rat skeletal muscle tissue, human colon cancer tissue, human fetal skeletal muscle tissue, mouse brain tissue, SH-SY5Y. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q06124 Human | P35235 Mouse | P41499 Rat |
| Alternative names: | BPTP3 CFC JMML METCDS MGC14433 NS1 OTTHUMP00000166107 OTTHUMP00000166108 Protein tyrosine phosphatase 2 Protein tyrosine phosphatase 2C Protein tyrosine phosphatase non receptor type 11 Protein-tyrosine phosphatase 1D Protein-tyrosine phosphatase 2C PTN11_HUMAN PTP-1D PTP-2C PTP1D PTP2C PTPN11 SAP2 SH-PTP2 SH-PTP3 SH2 domain containing protein tyrosine phosphatase 2 SHP 2 SHP-2 Shp2 SHPTP2 SHPTP3 Syp Tyrosine-protein phosphatase non-receptor type 11 |
Images
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Fig1:
ICC staining SHP2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining SHP2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining SHP2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-SHP2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cacner tissue using anti-SHP2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SHP2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SHP2 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of SH-SY5Y cells with SHP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.