Anti-IDO antibody
cat.: ER1706-94
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 45 kDa
Isotype: IgG
Immunogen: Recombinant protein within human IDO aa 176-342.
Positive control: Human placenta tissue lysate, HUVEC, Hela, human tonsil tissue, human spleen tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P14902 Human
Images
ER1706-94_1.jpg Fig1: Western blot analysis of IDO on human placenta tissue lysate using anti-IDO antibody at 1/500 dilution.
ER1706-94_2.jpg Fig2: ICC staining IDO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-94_3.jpg Fig3: ICC staining IDO in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-94_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IDO antibody. Counter stained with hematoxylin.
ER1706-94_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IDO antibody. Counter stained with hematoxylin.
ER1706-94_6.jpg Fig6: Flow cytometric analysis of HUVEC cells with IDO antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.