IDO Rabbit Polyclonal Antibody
cat.: ER1706-94
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IDO aa 176-342. |
| Positive control: | A549 treated with 50ng/mL IFN-gamma for 24 hours cell lysate, A549 cells treated with 50ng/mL IFN-gamma for 24 hours, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P14902 Human | P28776 Mouse |
| Alternative names: | 3-dioxygenase I23O1_HUMAN IDO 1 IDO IDO-1 IDO1 INDO indolamine 2,3 dioxygenase Indole 2 3 dioxygenase indoleamine 2 3 dioxygenase 1 indoleamine 2 3 dioxygenase Indoleamine 2,3-dioxygenase 1 Indoleamine pyrrole 2 3 dioxygenase Indoleamine-pyrrole 2 |
Images
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Fig1:
Western blot analysis of IDO on different lysates with Rabbit anti-IDO antibody (ER1706-94) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with 50ng/mL IFN-gamma for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A549 cells untreated / treated with 50ng/mL IFN-gamma for 24 hours labeling IDO with Rabbit anti-IDO antibody (ER1706-94) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IDO antibody (ER1706-94) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IDO antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IDO antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of HUVEC cells with IDO antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.