EMC10 Rabbit Polyclonal Antibody
cat.: ER1706-95
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse EMC10 aa 1-258 / 258. |
| Positive control: | A549, human breast tissue, human kidney tissue, mouse testis tissue. |
| Subcellular location: | Secreted, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:400 |
| Uniprot #: | SwissProt: Q5UCC4 Human | Q3TAS6 Mouse |
| Alternative names: | Chromosome 19 open reading frame 63 emc10 EMC10_HUMAN ER membrane protein complex subunit 10 Hematopoietic signal peptide containing membrane domain containing 1 Hematopoietic signal peptide containing membrane domain containing protein 1 Hematopoietic signal peptide containing secreted 1 Hematopoietic signal peptide-containing membrane domain-containing protein 1 HSM1 HSS1 INM02 UPF0510 protein INM02 |
Images
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Fig1:
Western blot analysis of EMC10 on A549 cell lysate using anti-EMC10 antibody at 1/500 dilution. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-EMC10 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EMC10 antibody. Counter stained with hematoxylin. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-EMC10 antibody (ER1706-95) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-95) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.