PFAS Rabbit Polyclonal Antibody
cat.: ER1706-96
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 145 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PURL aa 1,133-1,338 / 1,338. |
| Positive control: | 293T cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Hela cell lysate, HepG2 cell lysate, A549 cell lysate, 293 cell lysate, human tonsil tissue, human spleen tissue, human breast tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: O15067 Human | Q5SUR0 Mouse |
| Alternative names: | PFAS FGAM synthase FGAMS FGAR amidotransferase FGARAT Formylglycinamide ribotide amidotransferase Formylglycinamide ribotide synthetase KIAA0361 Phosphoribosylformylglycinamidine synthase PURL |
Images
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Fig1:
Western blot analysis of PFAS on different lysates with Rabbit anti-PFAS antibody (ER1706-96) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Hela cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: 293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 145 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-96) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PFAS antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PFAS antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PFAS antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PFAS antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.