PFAS Rabbit Polyclonal Antibody
cat.: ER1706-96
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 145 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PURL aa 1,133-1,338 / 1,338. |
| Positive control: | 293T cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Hela cell lysate, HepG2 cell lysate, A549 cell lysate,NIH/3T3 cell lysate, human tonsil tissue, human spleen tissue, human breast tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: O15067 Human | Q5SUR0 Mouse |
| Alternative names: | PFAS FGAM synthase FGAMS FGAR amidotransferase FGARAT Formylglycinamide ribotide amidotransferase Formylglycinamide ribotide synthetase KIAA0361 Phosphoribosylformylglycinamidine synthase PURL |
Images
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Fig1:
Western blot analysis of PFAS on different lysates with Rabbit anti-PFAS antibody (ER1706-96) at 1/5,000 dilution. Lane 1: 293T (Human embryonic kidney cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 4: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 5: A549 (Human lung adenocarcinoma cell) Lane 6: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-96, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 145 kDa Observed band size: 145 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PFAS antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PFAS antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PFAS antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PFAS antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.