ER1802-10

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 84 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within Human PSMA aa 676-725 / 750.

Positive control:

LNCaP cell lysates, HepG2, PC-3M.

Subcellular location:

Cytoplasm, Cell membrane.

Recommended Dilutions:
  WB
  IF-Cell
  FC

1:500-1:1,000
1:50-1:200
1:50-1:100

Uniprot #:

SwissProt: Q04609 Human

Alternative names:

Cell growth inhibiting protein 27 Cell growth-inhibiting gene 27 protein FGCP Folate hydrolase (prostate-specific membrane antigen) 1 Folate hydrolase 1 Folate hydrolase Folate hydrolase prostate specific membrane antigen 1 FOLH 1 FOLH Folh1 FOLH1_HUMAN Folylpoly gamma glutamate carboxypeptidase Folylpoly-gamma-glutamate carboxypeptidase GCP 2 GCP II GCP2 GCPII GIG27 Glutamate carboxylase II Glutamate carboxypeptidase 2 Glutamate carboxypeptidase II Membrane glutamate carboxypeptidase mGCP N acetylated alpha linked acidic dipeptidase 1 N-acetylated-alpha-linked acidic dipeptidase I NAALAD 1 NAALAD1 NAALAdase NAALADase I Prostate specific membrane antigen Prostate specific membrane antigen variant F Prostate-specific membrane antigen PSM PSMA Pteroylpoly gamma glutamate carboxypeptidase Pteroylpoly-gamma-glutamate carboxypeptidase

	Fig1: Western blot analysis of PSMA on LNCaP cell lysates with Rabbit anti-PSMA antibody (ER1802-10) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 84 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-10) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
	Fig2: ICC staining PSMA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining PSMA in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Flow cytometric analysis of HepG2 cells with PSMA antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).