PSMA Rabbit Polyclonal Antibody
cat.: ER1802-10
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 84 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PSMA aa 676-725 / 750.
Positive control: LNCaP cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate.
Subcellular location: Cytoplasm, Cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q04609 Human | O35409 Mouse | P70627 Rat
Alternative names: Cell growth inhibiting protein 27 Cell growth-inhibiting gene 27 protein FGCP Folate hydrolase (prostate-specific membrane antigen) 1 Folate hydrolase 1 Folate hydrolase Folate hydrolase prostate specific membrane antigen 1 FOLH 1 FOLH Folh1 FOLH1_HUMAN Folylpoly gamma glutamate carboxypeptidase Folylpoly-gamma-glutamate carboxypeptidase GCP 2 GCP II GCP2 GCPII GIG27 Glutamate carboxylase II Glutamate carboxypeptidase 2 Glutamate carboxypeptidase II Membrane glutamate carboxypeptidase mGCP N acetylated alpha linked acidic dipeptidase 1 N-acetylated-alpha-linked acidic dipeptidase I NAALAD 1 NAALAD1 NAALAdase NAALADase I Prostate specific membrane antigen Prostate specific membrane antigen variant F Prostate-specific membrane antigen PSM PSMA Pteroylpoly gamma glutamate carboxypeptidase Pteroylpoly-gamma-glutamate carboxypeptidase
Images
ER1802-10_1.jpg Fig1: Western blot analysis of PSMA on different lysates with Rabbit anti-PSMA antibody (ER1802-10) at 1/1,000 dilution.

Lane 1: LNCaP cell lysate (20 µg/Lane)
Lane 2: PC-3 cell lysate (20 µg/Lane) (negative)
Lane 3: Mouse kidney tissue lysate (40 µg/Lane)
Lane 4: Rat kidney tissue lysate (40 µg/Lane)

Predicted band size: 84 kDa
Observed band size: 110 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.