LSD1 Rabbit Polyclonal Antibody
cat.: ER1802-12
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 110 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human LSD1.
Positive control: PC-12, SiHa, A431, HepG2, SH-SY-5Y, rat kidney tissue, human colon cancer tissue, human spleen tissue, human kidney tissue, mouse cerebellum tissue, K562.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1,000
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O60341 Human | Q6ZQ88 Mouse
Alternative names: Amine oxidase (flavin containing) domain 2 Amine oxidase, flavin containing, 2 AOF2 BHC110 BRAF35 HDAC complex protein BHC110 BRAF35-HDAC complex protein BHC110 BRAF35/HDAC complex, 110-kD subunit CPRF EC1 FAD binding protein BRAF35 HDAC complex, 110 kDa subunit Flavin-containing amine oxidase domain-containing protein 2 KDM 1 KDM1 Kdm1a KDM1A_HUMAN KIAA0601 LSD 1 LSD1 Lysine (K) specific demethylase 1 Lysine (K) specific demethylase 1A Lysine demethylase 1A Lysine specific histone demethylase 1 Lysine specific histone demethylase 1A Lysine-specific demethylase 1 Lysine-specific demethylase 1A Lysine-specific histone demethylase 1A
Images
ER1802-12_1.jpg Fig1: Western blot analysis of LSD1 on PC-12 and SiHa cell lysates using anti-LSD1 antibody at 1/1,000 dilution.
ER1802-12_2.jpg Fig2: ICC staining LSD1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-12_3.jpg Fig3: ICC staining LSD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-12_4.jpg Fig4: ICC staining LSD1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-12_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LSD1 antibody. Counter stained with hematoxylin.
ER1802-12_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-LSD1 antibody. Counter stained with hematoxylin.
ER1802-12_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-LSD1 antibody. Counter stained with hematoxylin.
ER1802-12_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LSD1 antibody. Counter stained with hematoxylin.
ER1802-12_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-LSD1 antibody. Counter stained with hematoxylin.
ER1802-12_10.jpg Fig10: Flow cytometric analysis of K562 cells with LSD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.