LSD1 Rabbit Polyclonal Antibody
cat.: ER1802-12
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human LSD1. |
| Positive control: | PC-12 cell lysates, SiHa cell lysates, A431, HepG2, SH-SY-5Y, rat kidney tissue, human colon cancer tissue, human spleen tissue, human kidney tissue, mouse cerebellum tissue, K562. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1,000 1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O60341 Human | Q6ZQ88 Mouse |
| Alternative names: | Amine oxidase (flavin containing) domain 2 Amine oxidase, flavin containing, 2 AOF2 BHC110 BRAF35 HDAC complex protein BHC110 BRAF35-HDAC complex protein BHC110 BRAF35/HDAC complex, 110-kD subunit CPRF EC1 FAD binding protein BRAF35 HDAC complex, 110 kDa subunit Flavin-containing amine oxidase domain-containing protein 2 KDM 1 KDM1 Kdm1a KDM1A_HUMAN KIAA0601 LSD 1 LSD1 Lysine (K) specific demethylase 1 Lysine (K) specific demethylase 1A Lysine demethylase 1A Lysine specific histone demethylase 1 Lysine specific histone demethylase 1A Lysine-specific demethylase 1 Lysine-specific demethylase 1A Lysine-specific histone demethylase 1A |
Images
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Fig1:
Western blot analysis of LSD1 on different lysates with Rabbit anti-LSD1 antibody (ER1802-12) at 1/1,000 dilution. Lane 1: PC-12 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 100/80 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-12) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LSD1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-LSD1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-LSD1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LSD1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-LSD1 antibody. Counter stained with hematoxylin. |
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Fig7: ICC staining LSD1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig8: ICC staining LSD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig9: ICC staining LSD1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig10: Flow cytometric analysis of K562 cells with LSD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.