| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Beta-Arrestin 1. |
| Positive control: | Human placenta tissue lysate, A549, HUVEC, LOVO, rat brain tissue, human colon cancer tissue, human breast tissue, mouse colon tissue, Jurkat. |
| Subcellular location: | Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1000 1:50-1:200 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P49407 Human | Q8BWG8 Mouse | P29066 Rat |
| Alternative names: | ARB1 ARR1 ARRB1 ARRB1_HUMAN Arrestin 2 Arrestin beta 1 Arrestin beta-1 Beta-arrestin-1 |
|
Fig1:
Western blot analysis of beta Arrestin 1 on human placenta tissue lysate using anti-beta Arrestin 1 antibody at 1/500 dilution. |
|
Fig2: ICC staining beta Arrestin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining beta Arrestin 1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining beta Arrestin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin. |
|
Fig8: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin. |
|
Fig9: Flow cytometric analysis of Jurkat cells with beta Arrestin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |