Mitofusin 2 Rabbit Polyclonal Antibody
cat.: ER1802-23
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 86 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Mitofusin 2 aa 378-602.
Positive control: Rat heart tissue lysates, A431, LOVO, PC-3M, Jurkat.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O95140 Human | Q8R500 Rat
Alternative names: CMT2A CMT2A2 CPRP 1 CPRP1 EC 3.6.5.- Fzo HSG hyperplasia suppressor gene Hypertension related protein 1 KIAA0214 MARF MFN 2 Mfn2 MFN2_HUMAN Mitochondrial assembly regulatory factor Mitofusin-2 Mitofusin2 Transmembrane GTPase MFN2
Images
ER1802-23_1.jpg Fig1: Western blot analysis of Mitofusin 2 on rat heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1802-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ER1802-23_2.jpg Fig2: ICC staining of Mitofusin 2 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1802-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1802-23_3.jpg Fig3: ICC staining of Mitofusin 2 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1802-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1802-23_4.jpg Fig4: ICC staining of Mitofusin 2 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1802-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1802-23_5.jpg Fig5: Flow cytometric analysis of Mitofusin 2 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1802-23, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.