SATB1 Rabbit Polyclonal Antibody
cat.: ER1802-24
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 86 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human SATB1 aa. |
| Positive control: | Mouse thymus tissue lysate,SH-SY-5Y, Jurkat. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q01826 Human | Q60611 Mouse |
| Alternative names: | DNA binding protein SATB1 DNA-binding protein SATB1 SATB homeobox 1 SATB1 SATB1_HUMAN Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) Special AT rich sequence binding protein 1 Special AT-rich sequence-binding protein 1 |
Images
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Fig1: ICC staining SATB1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig2:
Western blot analysis of SATB1 on different lysates with Rabbit anti-SATB1 antibody (ER1802-24) at 1/1,000 dilution. Lane 1: THP-1(Human acute monoblastic leukemia cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-24, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 86 kDa Observed band size: 86 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.