USP21 Rabbit Polyclonal Antibody
cat.: ER1802-25
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 63 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human USP21 aa 1-50 / 565. |
| Positive control: | A549 cell lysate, HepG2 cell lysate, PC-3M, SH-SY-5Y, human liver cancer tissue, human kidney tissue, human fetal skeletal muscle tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5000 1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UK80 Human | Q9QZL6 Mouse | B2GUX4 Rat |
| Alternative names: | Deubiquitinating enzyme 21 deubiquitinating enzyme 23 EC 3.1.2.15 ESTM28 MGC3394 NEDD8-specific protease Ubiquitin carboxyl terminal hydrolase 21 ubiquitin carboxyl terminal hydrolase 23 Ubiquitin carboxyl-terminal hydrolase 21 ubiquitin specific peptidase 21 Ubiquitin specific processing protease 21 ubiquitin specific protease 16 ubiquitin specific protease 21 ubiquitin specific protease 23 Ubiquitin thioesterase 21 Ubiquitin thiolesterase 21 ubiquitin thiolesterase 23 ubiquitin-specific protease 23, formerly Ubiquitin-specific-processing protease 21 UBP21_HUMAN USP16 v Usp21 USP23 USP23, formerly W53272 |
Images
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Fig1:
Western blot analysis of USP21 on different lysates with Rabbit anti-USP21 antibody (ER1802-25) at 1/5,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-25, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 63 kDa Observed band size: 59 kDa |
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Fig2: ICC staining USP21 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining USP21 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-USP21 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-USP21 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-USP21 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.