Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 70/55 kDa |
Isotype: | IgG |
Immunogen: | Native protein. |
Positive control: | Mouse bone marrow and human kidney tissue lysates, A549, NIH-3T3. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB IF-Cell FC |
1:500 1:100-1:500 1:50-1:100 |
Uniprot #: | SwissProt: P02671 | P0267 Human 5 | P02679 Human |
Alternative names: | FGA Fib2 FIBA_HUMAN Fibrinogen A alpha polypeptide Fibrinogen alpha chain Fibrinogen B alpha polypeptide Fibrinogen beta chain Fibrinogen G alpha polypeptide Fibrinogen gamma chain fibrinogen, B beta polypeptide fibrinogen, G gamma polypeptide fibrinogen, gamma polypeptide Fibrinogen--alpha -polypeptide chain Fibrinogen--beta -polypeptide chain Fibrinogen--gamma-polypeptide chain |
Fig1: Western blot analysis of Fibrinogen on mouse bone marrow and human kidney tissue lysates using anti-Fibrinogen antibody at 1/500 dilution. | |
Fig2: ICC staining Fibrinogen in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Fibrinogen in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Flow cytometric analysis of NIH-3T3 cells with Fibrinogen antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |