Fibrinogen Rabbit Polyclonal Antibody
cat.: ER1802-26
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 70/55 kDa |
| Isotype: | IgG |
| Immunogen: | Native protein. |
| Positive control: | Hep G2 cell lysate, human plasma lysate, A549, NIH-3T3, human placenta tissue, human lung tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:1,000-1:5,000 1:100-1:500 1:50-1:100 1:8,000 |
| Uniprot #: | SwissProt: P02671 Human | P02675 Human | P02679 Human |
| Alternative names: | FGA Fib2 FIBA_HUMAN Fibrinogen A alpha polypeptide Fibrinogen alpha chain Fibrinogen B alpha polypeptide Fibrinogen beta chain Fibrinogen G alpha polypeptide Fibrinogen gamma chain fibrinogen, B beta polypeptide fibrinogen, G gamma polypeptide fibrinogen, gamma polypeptide Fibrinogen--alpha -polypeptide chain Fibrinogen--beta -polypeptide chain Fibrinogen--gamma-polypeptide chain |
Images
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Fig1:
Western blot analysis of Fibrinogen on different lysates with Rabbit anti-Fibrinogen antibody (ER1802-26) at 1/5,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: Human plasma lysate Lysates/proteins at 10 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-26, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50-100 kDa Observed band size: 50-100 kDa |
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Fig2: ICC staining Fibrinogen in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Fibrinogen in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Fibrinogen antibody (ER1802-26) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-26) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Fibrinogen antibody (ER1802-26) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-26) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.