Fibrinogen Rabbit Polyclonal Antibody
cat.: ER1802-26
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 70/55 kDa |
| Isotype: | IgG |
| Immunogen: | Native protein. |
| Positive control: | Mouse bone marrow tissue lysates, human kidney tissue lysates, A549, NIH-3T3, human placenta tissue, human lung tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:500 1:100-1:500 1:50-1:100 1:8,000 |
| Uniprot #: | SwissProt: P02671 | P0267 Human 5 | P02679 Human |
| Alternative names: | FGA Fib2 FIBA_HUMAN Fibrinogen A alpha polypeptide Fibrinogen alpha chain Fibrinogen B alpha polypeptide Fibrinogen beta chain Fibrinogen G alpha polypeptide Fibrinogen gamma chain fibrinogen, B beta polypeptide fibrinogen, G gamma polypeptide fibrinogen, gamma polypeptide Fibrinogen--alpha -polypeptide chain Fibrinogen--beta -polypeptide chain Fibrinogen--gamma-polypeptide chain |
Images
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Fig1: Western blot analysis of Fibrinogen on mouse bone marrow and human kidney tissue lysates using anti-Fibrinogen antibody at 1/500 dilution. |
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Fig2: ICC staining Fibrinogen in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Fibrinogen in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Flow cytometric analysis of NIH-3T3 cells with Fibrinogen antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Fibrinogen antibody (ER1802-26) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-26) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Fibrinogen antibody (ER1802-26) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-26) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.