ER1802-28

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Rat
Applications:	WB, IF-Cell, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

45 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within N-terminal human CXCR4 aa.

Positive control:

293, K562, Hela, HUVEC, THP-1.

Subcellular location:

Cell junction. Plasma membrane. Endosome. Lysosome.

Recommended Dilutions:
  WB
  IF-Cell
  FC

1:500-1:1,000
1:500-1:2,000
1:50-1:100

Uniprot #:

SwissProt: P61073 Human | O08565 Rat

Alternative names:

C-X-C chemokine receptor type 4 CD184 CD184 antigen Chemokine (C X C motif) receptor 4 Chemokine CXC Motif Receptor 4 CXC-R4 CXCR-4 CXCR4 CXCR4_HUMAN D2S201E FB22 Fusin HM89 HSY3RR LAP 3 LAP3 LCR1 LESTR Leukocyte derived seven transmembrane domain receptor Leukocyte-derived seven transmembrane domain receptor Lipopolysaccharide associated protein 3 Neuropeptide Y receptor Y3 NPY3R NPYR NPYRL NPYY3 NPYY3R Probable G protein coupled receptor lcr1 homolog SDF 1 receptor SDF-1 receptor SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR Stromal cell derived factor 1 receptor Stromal cell-derived factor 1 receptor WHIM WHIMS

	Fig1: Western blot analysis of CXCR4 on different cell lysates using anti-CXCR4 antibody at 1/500 dilution. Positive control: Lane 1: 293 Lane 2: K562
	Fig2: ICC staining CXCR4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining CXCR4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Flow cytometric analysis of THP-1 cells with CXCR4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.