CXCR4 Rabbit Polyclonal Antibody
cat.: ER1802-28
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 40 kDa
Isotype: IgG
Immunogen: KLH conjugated synthetic peptide derived from the middle of human CXCR4
Positive control: 293, K562, Hela, HUVEC, THP-1.
Subcellular location: Cell membrane; Multi-pass membrane protein.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
1:500-2000
1:100-500
1:100-500
Uniprot #: SwissProt: P61073 Human | O08565 Rat
Alternative names: C-X-C chemokine receptor type 4 CD184 CD184 antigen Chemokine (C X C motif) receptor 4 Chemokine CXC Motif Receptor 4 CXC-R4 CXCR-4 CXCR4 CXCR4_HUMAN D2S201E FB22 Fusin HM89 HSY3RR LAP 3 LAP3 LCR1 LESTR Leukocyte derived seven transmembrane domain receptor Leukocyte-derived seven transmembrane domain receptor Lipopolysaccharide associated protein 3 Neuropeptide Y receptor Y3 NPY3R NPYR NPYRL NPYY3 NPYY3R Probable G protein coupled receptor lcr1 homolog SDF 1 receptor SDF-1 receptor SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR Stromal cell derived factor 1 receptor Stromal cell-derived factor 1 receptor WHIM WHIMS
Images
ER1802-28_1.jpg Fig1: Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Lane 3: Bone (Rat) Lysate at 40 ug
Lane 4: Spleen (Rat) Lysate at 40 ug
Primary: Anti-CXCR4 (bs-20317R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 44 kD
ER1802-28_2.jpg Fig2: Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (ER1802-28) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
ER1802-28_3.jpg Fig3: Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (ER1802-28) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
ER1802-28_4.jpg Fig4: Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (ER1802-28) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
ER1802-28_5.jpg Fig5: Paraformaldehyde-fixed, paraffin embedded Human prostate tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (ER1802-28) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
ER1802-28_6.jpg Fig6: Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (ER1802-28) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
ER1802-28_7.jpg Fig7: Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (ER1802-28) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
ER1802-28_8.jpg Fig8: Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (ER1802-28) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
ER1802-28_9.jpg Fig9: Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (ER1802-28) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.