Apolipoprotein A1 Rabbit Polyclonal Antibody
cat.: ER1802-29
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Apolipoprotein A1. |
| Positive control: | Human liver tissue, HepG2, LOVO, SH-SY-5Y, rat liver tissue, mouse liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P02647 Human | Q00623 Mouse | P04639 Rat |
| Alternative names: | Apo-AI ApoA I ApoA-I APOA1 APOA1_HUMAN Apolipoprotein A-I(1-242) Apolipoprotein A1 Apolipoprotein AI Brp14 Ltw1 Lvtw1 Sep1 Sep2 |
Images
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Fig1: Western blot analysis of Apolipoprotein A1 on human liver tissue lysate using anti-Apolipoprotein A1 antibody at 1/1,000 dilution. |
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Fig2: ICC staining Apolipoprotein A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Apolipoprotein A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Apolipoprotein A I in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Apolipoprotein A1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Apolipoprotein A1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein A I antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of HepG2 cells with Apolipoprotein A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.