SFRP1 Rabbit Polyclonal Antibody
cat.: ER1802-34
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human SFRP1. |
| Positive control: | A549, SH-SY-5Y, rat testis tissue, human colon cancer tissue, human breast tissue, human placenta tissue, mouse small intestine tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q8N474 Human | Q8C4U3 Mouse | Q9R168 Rat |
| Alternative names: | Frizzled related protein 1 FRP 1 FRP FRP-1 FRP1 FrzA SARP 2 SARP-2 SARP2 Secreted apoptosis related protein 2 Secreted apoptosis-related protein 2 Secreted frizzled related protein 1 Secreted frizzled related protein Secreted frizzled-related protein 1 SFRP 1 sFRP-1 SFRP1 SFRP1_HUMAN |
Images
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Fig1: ICC staining SFRP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining SFRP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SFRP1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SFRP1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SFRP1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SFRP1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-SFRP1 antibody. Counter stained with hematoxylin. |
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Fig8:
Western blot analysis of SFRP1 on different lysates with Rabbit anti-SFRP1 antibody (ER1802-34) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate （20 µg/Lane） Lane 2: SK-MEL-28 cell lysate（20 µg/Lane） Lane 3: NIH/3T3 cell lysate（20 µg/Lane） Lane 4: mouse kidney tissue lysate（40 µg/Lane） Lane 5: mouse testis tissue lysate（40 µg/Lane） Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-34, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 35.4 kDa Observed band size: 35 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.