PTP1B Rabbit Polyclonal Antibody
cat.: ER1802-35
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 50 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PTP1B aa 1-191 / 435.
Positive control: Recombinant protein lysate, LOVO, SiHa, human colon cancer tissue, human placenta tissue, mouse fallopian tubes tissue.
Subcellular location: Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:1,000-1;5,000
1:500-1:2,000
1:100-1:500
Uniprot #: SwissProt: P18031 Human | P35821 Mouse
Alternative names: PTP1B Non receptor tyrosine phosphatase 1 Protein phosphotyrosylphosphatase 1B Protein tyrosine phosphatase 1B Protein tyrosine phosphatase non receptor type 1 Protein tyrosine phosphatase placental Protein-tyrosine phosphatase 1B PTN1_HUMAN PTP 1B PTP-1B PTPN 1 PTPN1 Tyrosine protein phosphatase non receptor type 1 Tyrosine-protein phosphatase non-receptor type 1
Images
ER1802-35_1.jpg Fig1: Western blot analysis of PTP1B on recombinant protein lysate using anti-PTP1B antibody at 1/5,000 dilution.
ER1802-35_2.jpg Fig2: ICC staining PTP1B in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-35_3.jpg Fig3: ICC staining PTP1B in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-35_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
ER1802-35_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
ER1802-35_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.