Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 50 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human PTP1B aa 1-191 / 435. |
Positive control: | Recombinant protein lysate, LOVO, SiHa, human colon cancer tissue, human placenta tissue, mouse fallopian tubes tissue. |
Subcellular location: | Endoplasmic reticulum membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1;5,000 1:500-1:2,000 1:100-1:500 |
Uniprot #: | SwissProt: P18031 Human | P35821 Mouse |
Alternative names: | PTP1B Non receptor tyrosine phosphatase 1 Protein phosphotyrosylphosphatase 1B Protein tyrosine phosphatase 1B Protein tyrosine phosphatase non receptor type 1 Protein tyrosine phosphatase placental Protein-tyrosine phosphatase 1B PTN1_HUMAN PTP 1B PTP-1B PTPN 1 PTPN1 Tyrosine protein phosphatase non receptor type 1 Tyrosine-protein phosphatase non-receptor type 1 |
Fig1: Western blot analysis of PTP1B on recombinant protein lysate using anti-PTP1B antibody at 1/5,000 dilution. | |
Fig2: ICC staining PTP1B in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining PTP1B in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTP1B antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PTP1B antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-PTP1B antibody. Counter stained with hematoxylin. |