Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | IHC-P, FC, WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 31 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within C-terminal human IL1 alpha. |
Positive control: | Human lymph node lysates, Human colon cancer tissue, PC-3M. |
Subcellular location: | Nucleus, Cytoplasm, Secreted. |
Recommended Dilutions:
IHC-P FC WB |
1:400-1:800 1:50-1:100 1:500 |
Uniprot #: | SwissProt: P01583 Human | P01582 Mouse |
Alternative names: | BAF FAF Hematopoietin 1 Hematopoietin-1 IL 1 alpha IL 1A IL-1 alpha Il-1a IL1 ALPHA IL1 IL1A IL1A_HUMAN IL1F1 Interleukin 1 alpha Interleukin-1 alpha Interleukin1 alpha LAF LEM Preinterleukin 1 alpha Pro interleukin 1 alpha |
Fig1: Western blot analysis of IL1 alpha on human lymph node lysates using anti-IL1 alpha antibody at 1/500 dilution. | |
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IL1 alpha antibody. Counter stained with hematoxylin. | |
Fig3: Flow cytometric analysis of PC-3M cells with IL1 alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |