Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 99/82 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human Progesterone Receptor aa 522-567. |
Positive control: | Mouse smooth muscle tissue lysate, A549, Hela, MCF-7. |
Subcellular location: | Nucleus. Cytoplasm. |
Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:200-1:800 1:50-1:100 |
Uniprot #: | SwissProt: P06401 Human | Q00175 Mouse |
Alternative names: | NR3C3 Nuclear receptor subfamily 3 group C member 3 PGR PR PRA PRB PRGR_HUMAN Progesterone receptor Progestin receptor form A Progestin receptor form B |
Fig1: Western blot analysis of Progesterone Receptor on mouse smooth muscle tissue lysate using anti-Progesterone Receptor antibody at 1/500 dilution. | |
Fig2: ICC staining Progesterone Receptor in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Progesterone Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining Progesterone Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Flow cytometric analysis of MCF-7 cells with Progesterone Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |