| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 99/82 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Progesterone Receptor aa 522-567. |
| Positive control: | Mouse smooth muscle tissue lysate, A549, Hela, MCF-7. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:200-1:800 1:50-1:100 |
| Uniprot #: | SwissProt: P06401 Human | Q00175 Mouse |
| Alternative names: | NR3C3 Nuclear receptor subfamily 3 group C member 3 PGR PR PRA PRB PRGR_HUMAN Progesterone receptor Progestin receptor form A Progestin receptor form B |
|
Fig1: Western blot analysis of Progesterone Receptor on mouse smooth muscle tissue lysate using anti-Progesterone Receptor antibody at 1/500 dilution. |
|
Fig2: ICC staining Progesterone Receptor in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Progesterone Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining Progesterone Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Flow cytometric analysis of MCF-7 cells with Progesterone Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |