Progesterone Receptor Rabbit Polyclonal Antibody
cat.: ER1802-37
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 99/82 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Progesterone Receptor aa 522-567.
Positive control: Mouse smooth muscle tissue lysate, A549, Hela, MCF-7.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  FC

1:500-1:1,000
1:200-1:800
1:50-1:100
Uniprot #: SwissProt: P06401 Human | Q00175 Mouse
Alternative names: NR3C3 Nuclear receptor subfamily 3 group C member 3 PGR PR PRA PRB PRGR_HUMAN Progesterone receptor Progestin receptor form A Progestin receptor form B
Images
ER1802-37_1.jpg Fig1: Western blot analysis of Progesterone Receptor on mouse smooth muscle tissue lysate using anti-Progesterone Receptor antibody at 1/500 dilution.
ER1802-37_2.jpg Fig2: ICC staining Progesterone Receptor in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-37_3.jpg Fig3: ICC staining Progesterone Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-37_4.jpg Fig4: ICC staining Progesterone Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-37_5.jpg Fig5: Flow cytometric analysis of MCF-7 cells with Progesterone Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.