Aromatase Rabbit Polyclonal Antibody
cat.: ER1802-38
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 58 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Aromatase aa 91-140 / 503.
Positive control: JAR cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, rat placenta tissue lysate, HepG2, SH-SY-5Y, SK-Br-3, HeLa, human placenta tissue.
Subcellular location: Endoplasmic reticulum membrane, Microsome membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000
1:50-1:200
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: P11511 Human | P28649 Mouse | P22443 Rat
Alternative names: ARO ARO1 Aromatase CP19A_HUMAN CPV1 CYAR CYP19 Cyp19a1 CYPXIX Cytochrome P-450AROM Cytochrome P450 19A1 Cytochrome P450, family 19, subfamily A, polypeptide 1 Cytochrome P450, subfamily XIX (aromatization of androgens) Estrogen synthase Estrogen synthetase Flavoprotein linked monooxygenase MGC104309 Microsomal monooxygenase OTTHUMP00000162543 OTTHUMP00000198350 P 450AROM
Images
ER1802-38_1.jpg Fig1: Western blot analysis of Aromatase on different lysates with Rabbit anti-Aromatase antibody (ER1802-38) at 1/1,000 dilution.

Lane 1: JAR cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: Rat placenta tissue lysate (30 µg/Lane)

Predicted band size: 58 kDa
Observed band size: 58 kDa

Exposure time: Lane 1-3: 14 seconds; Lane 4: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1802-38_2.jpg Fig2: ICC staining Aromatase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-38_3.jpg Fig3: ICC staining Aromatase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-38_4.jpg Fig4: ICC staining Aromatase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-38_5.png Fig5: Immunocytochemistry analysis of MDA-MB-468 cells labeling Aromatase with Rabbit anti-Aromatase antibody (ER1802-38) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Aromatase antibody (ER1802-38) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ER1802-38_6.png Fig6: Immunocytochemistry analysis of Hela cells labeling Aromatase with Rabbit anti-Aromatase antibody (ER1802-38) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Aromatase antibody (ER1802-38) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ER1802-38_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Aromatase antibody. Counter stained with hematoxylin.
ER1802-38_8.jpg Fig8: Flow cytometric analysis of HepG2 cells with Aromatase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.