Aromatase Rabbit Polyclonal Antibody
cat.: ER1802-38
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Aromatase aa 91-140 / 503. |
| Positive control: | Jurkat, HL-60, HepG2, SH-SY-5Y, SK-Br-3, Hela, MDA-MB-468, human placenta tissue, mouse cerebellum tissue lysate. |
| Subcellular location: | Endoplasmic reticulum membrane, Microsome membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P11511 Human | P28649 Mouse |
| Alternative names: | ARO ARO1 Aromatase CP19A_HUMAN CPV1 CYAR CYP19 Cyp19a1 CYPXIX Cytochrome P-450AROM Cytochrome P450 19A1 Cytochrome P450, family 19, subfamily A, polypeptide 1 Cytochrome P450, subfamily XIX (aromatization of androgens) Estrogen synthase Estrogen synthetase Flavoprotein linked monooxygenase MGC104309 Microsomal monooxygenase OTTHUMP00000162543 OTTHUMP00000198350 P 450AROM |
Images
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Fig1: Western blot analysis of Aromatase on mouse cerebellum tissue lysate using anti-Aromatase antibody at 1/500 dilution. |
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Fig2:
Western blot analysis of Aromatase on different lysates with Rabbit anti-Aromatase antibody (ER1802-38) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-38) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining Aromatase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Aromatase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining Aromatase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6:
Immunocytochemistry analysis of MDA-MB-468 cells labeling Aromatase with Rabbit anti-Aromatase antibody (ER1802-38) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Aromatase antibody (ER1802-38) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig7:
Immunocytochemistry analysis of Hela cells labeling Aromatase with Rabbit anti-Aromatase antibody (ER1802-38) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Aromatase antibody (ER1802-38) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Aromatase antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of HepG2 cells with Aromatase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.