APC Rabbit Polyclonal Antibody
cat.: ER1802-39
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 312 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human Ifna1.
Positive control: HUVEC, SH-SY-5Y, SiHa, rat brain tissue, human kidney tissue, human breast tissue, mouse colon tissue.
Subcellular location: Cell junction. Cytoplasm. Cytoskeleton. Membrane.
Recommended Dilutions:
  ICC
  IHC-P
  FC
  WB

1:50-1:200
1:100-1:200
1:50-1:100
1:500
Uniprot #: SwissProt: P25054 Human | Q61315 Mouse | P70478 Rat
Alternative names: Adenomatous Polyposis Coli Adenomatous polyposis coli protein Apc APC_HUMAN CC1 Deleted in polyposis 2.5 DP2 DP2.5 DP3 FAP FPC GS Protein APC
Images
ER1802-39_1.jpg Fig1: ICC staining APC in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-39_2.jpg Fig2: ICC staining APC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-39_3.jpg Fig3: ICC staining APC in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-39_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-APC antibody. Counter stained with hematoxylin.
ER1802-39_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-APC antibody. Counter stained with hematoxylin.
ER1802-39_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-APC antibody. Counter stained with hematoxylin.
ER1802-39_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-APC antibody. Counter stained with hematoxylin.
ER1802-39_8.jpg Fig8: Flow cytometric analysis of SH-SY-5Y cells with APC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.