Interferon alpha Rabbit Polyclonal Antibody
cat.: ER1802-4
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse IFNA1 aa 140-181.
Positive control: Mouse liver tissue lysate, N2A, SHG-44, SH-SY5Y, PC-3M, rat brain tissue, human kidney tissue, mouse brain tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P01562 Human | P01572 Mouse | P05011 Rat
Alternative names: IFL IFN alpha IFN alpha 1/13 IFN alpha 1b IFN alphaD IFN IFN-alpha-1/13 IFNA@ IFNA1 IFNA1_HUMAN IFNA13 Interferon alpha 1 Interferon alpha-1/13 Interferon alpha-D Interferon alpha1 Interferon, alpha 13 LeIF D
Images
ER1802-4_1.jpg Fig1: Western blot analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.
ER1802-4_2.png Fig2: Immunocytochemistry analysis of PC-3M cells labeling Interferon alpha with Rabbit anti-Interferon alpha antibody (ER1802-4) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Interferon alpha antibody (ER1802-4) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

β-tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
ER1802-4_3.jpg Fig3: ICC staining IFNA1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-4_4.jpg Fig4: ICC staining IFNA1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-4_5.jpg Fig5: ICC staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-4_6.png Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
ER1802-4_7.png Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
ER1802-4_8.png Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
ER1802-4_9.jpg Fig9: Flow cytometric analysis of SH-SY5Y cells with IFNA1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
ER1802-4_10.jpg Fig10: Western blot analysis of Interferon alpha on mouse IFNA1 recombinant protein with Rabbit anti-Interferon alpha antibody (ER1802-4) at 1/5,000 dilution.

Lysates/proteins at 50 ng/Lane.

Exposure time: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-4) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.