HIF1 alpha Rabbit Polyclonal Antibody
cat.: ER1802-41
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Mouse, Rabbit, Pig, Cow, Dog
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 92 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human HIF1 alpha.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-cell
  FC
1:500-2000
1:100
1μg/Test: 1:5000-10000
Uniprot #: SwissProt: Q16665 Human | Q61221 Mouse
Alternative names: ARNT interacting protein ARNT-interacting protein Basic helix loop helix PAS protein MOP1 Basic-helix-loop-helix-PAS protein MOP1 bHLHe78 Class E basic helix-loop-helix protein 78 HIF 1A HIF 1alpha HIF-1-alpha HIF1 A HIF1 Alpha HIF1 HIF1-alpha HIF1A HIF1A_HUMAN Hypoxia inducible factor 1 alpha Hypoxia inducible factor 1 alpha isoform I.3 Hypoxia inducible factor 1 alpha subunit Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) Hypoxia inducible factor1alpha Hypoxia-inducible factor 1-alpha Member of PAS protein 1 Member of PAS superfamily 1 Member of the PAS Superfamily 1 MOP 1 MOP1 PAS domain-containing protein 8 PASD 8 PASD8
Images
ER1802-41_1.jpg Fig1: 50 ng RHHIF-1 Alpha-Trx-His protein (bs-42326P) per lane probed with HIF-1 Alpha polyclonal antibody respectively, unconjugated (bs-0737R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
ER1802-41_2.jpg Fig2: 25 ug total protein per lane of various lysates (see on figure) probed with HIF-1 Alpha polyclonal antibody, unconjugated (bs-0737R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
ER1802-41_3.jpg Fig3: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, Unconjugated (bs-0737R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
ER1802-41_4.jpg Fig4: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, Unconjugated (bs-0737R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
ER1802-41_5.jpg Fig5: Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti- HIF-1 Alpha antibody (bs-0737R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
ER1802-41_6.jpg Fig6: Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-HIF-1 Alpha antibody (bs-0737R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.