Cytokeratin 8 Rabbit Polyclonal Antibody
cat.: ER1802-43
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 53 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human Cytokeratin 8.
Positive control: Human small intestine tissue lysate, A431, A549, LOVO, MCF-7, rat epididymis tissue, human lung cancer tissue, human prostate tissue, mouse liver tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P05787 Human | P11679 Mouse | Q10758 Rat
Alternative names: CARD2 CK 8 CK-8 CK8 CYK8 CYKER Cytokeratin endo A Cytokeratin-8 DreK8 EndoA K2C8 K2C8_HUMAN K8 Keratin 8 Keratin type II cytoskeletal 8 Keratin, type II cytoskeletal 8 Keratin-8 KO Krt 2.8 KRT8 MGC118110 MGC174782 MGC53564 MGC85764 sb:cb186 Type-II keratin Kb8
Images
ER1802-43_1.jpg Fig1: Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/500 dilution.
Positive control:
Lane 1: Human small intestine
Lane 2: A431
ER1802-43_2.jpg Fig2: ICC staining Cytokeratin 8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-43_3.jpg Fig3: ICC staining Cytokeratin 8 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-43_4.jpg Fig4: ICC staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-43_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
ER1802-43_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
ER1802-43_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
ER1802-43_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
ER1802-43_9.jpg Fig9: Flow cytometric analysis of MCF-7 cells with Cytokeratin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.