Cytokeratin 8 Rabbit Polyclonal Antibody
cat.: ER1802-43
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Cytokeratin 8. |
| Positive control: | Human small intestine tissue lysate, A431, A549, LOVO, MCF-7, rat epididymis tissue, human lung cancer tissue, human prostate tissue, mouse liver tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P05787 Human | P11679 Mouse | Q10758 Rat |
| Alternative names: | CARD2 CK 8 CK-8 CK8 CYK8 CYKER Cytokeratin endo A Cytokeratin-8 DreK8 EndoA K2C8 K2C8_HUMAN K8 Keratin 8 Keratin type II cytoskeletal 8 Keratin, type II cytoskeletal 8 Keratin-8 KO Krt 2.8 KRT8 MGC118110 MGC174782 MGC53564 MGC85764 sb:cb186 Type-II keratin Kb8 |
Images
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Fig1:
Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/500 dilution. Positive control: Lane 1: Human small intestine Lane 2: A431 |
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Fig2: ICC staining Cytokeratin 8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Cytokeratin 8 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of MCF-7 cells with Cytokeratin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.