CRMP2 Rabbit Polyclonal Antibody
cat.: ER1802-45
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CRMP2 aa 90-320. |
| Positive control: | SH-SY-5Y, HUVEC, human tonsil tissue, human colon tissue, human breast cancer tissue. |
| Subcellular location: | Cytosol. Cytoskeleton. Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:10,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q16555 Human | O08553 Mouse |
| Alternative names: | Collapsin response mediator protein 2 Collapsin response mediator protein Collapsin response mediator protein hCRMP 2 CRAM CRMP 2 CRMP-2 CRMP2 DHPRP 2 DHPRP2 Dihydropyrimidinase 2 Dihydropyrimidinase like 2 Dihydropyrimidinase like 2 long form Dihydropyrimidinase related protein 2 Dihydropyrimidinase-related protein 2 DPYL 2 DPYL2 DPYL2_HUMAN DPYSL 2 Dpysl2 DRP-2 DRP2 Musunc 33 Musunc33 N2A3 TOAD 64 TOAD64 ULIP 2 protein ULIP-2 Ulip2 Unc-33-like phosphoprotein 2 |
Images
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Fig1:
Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (ER1802-45) at 1/5,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: HUVEC (Human umbilical vein endothelial cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-45, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 62.3 kDa Observed band size: 60-80 kDa |
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Fig2: ICC staining CRMP2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CRMP2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CRMP2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CRMP2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CRMP2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CRMP2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.