ER1802-46

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 42/49/60 kDa

Isotype:

IgG

Immunogen:

Recombinant protein within Human CD55 aa 130-381.

Positive control:

Human colon cancer, human placenta tissue, HeLa cell lysates.

Subcellular location:

Cell membrane. Secreted.

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:5,000
1:50-1:200
1:50-1:200

Uniprot #:

SwissProt: P08174 Human

Alternative names:

CD 55 CD55 CD55 antigen CD55 Cromer blood group system CD55 molecule (Cromer blood group) CD55 molecule CD55 molecule, decay accelerating factor for complement (Cromer blood group) Cd55a Complement decay accelerating factor Complement decay-accelerating factor Complement decay-accelerating factor, GPI-anchored CR CROM Cromer Blood Group antigen Cromer blood group system DAF Daf-GPI DAF_HUMAN Daf1 Dcay accelerating factor for complement (CD55, Cromer blood group system) Decay accelarating factor 1, isoform CRA_a Decay accelerating factor (GPI-form) Decay Accelerating Factor for Complement Decay accelerating factor GPI-form Decay accelerating factor soluble-form GPI-DAF TC

	Fig1: ICC staining CD55 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD55 antibody. Counter stained with hematoxylin.
	Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD55 antibody. Counter stained with hematoxylin.
	Fig4: Western blot analysis of CD55 on HeLa cell lysate with Rabbit anti-CD55 antibody (ER1802-46) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-46, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 41 kDa Observed band size: 80 kDa