GOT1 Rabbit Polyclonal Antibody
cat.: ER1802-48
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal Human GOT1. |
| Positive control: | K562, human liver tissue, human colon tissue, human heart tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P17174 Human |
| Alternative names: | CGI-141 GCT2 Germ cell tumor 2 Golgi transport 1 homolog B Golgi transport 1B GOLT1B GOT1 GOT1B_HUMAN hGOT1a Putative NF-kappa-B-activating protein 470 Vesicle transport protein GOT1B YMR292W |
Images
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Fig1:
Western blot analysis of GOT1 on different lysates with Rabbit anti-GOT1 antibody (ER1802-48) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-GOT1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-48) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of GOT1 on K562 cell lysate using anti-GOT1 antibody at 1/500 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GOT1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-GOT1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-GOT1 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of HepG2 cells with GOT1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.