APE1 Rabbit Polyclonal Antibody
cat.: ER1802-49
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 36 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human APE1 aa 2-270.
Positive control: HL-60, human skin tissue lysate, mouse placenta tissue lysate, MCF-7, HepG2, LOVO, PC-3M, human liver tissue, human colon tissue, human breast cancer tissue, A549.
Subcellular location: Nucleus, Cytoplasm, Endoplasmic reticulum.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:500-1:1,000
1:50-1:500
1:50-1:100
Uniprot #: SwissProt: P27695 Human | P28352 Mouse | P43138 Rat
Alternative names: AP endonuclease 1 AP endonuclease class I AP lyase APE 1 APE APE-1 APEN APEX 1 APEX APEX nuclease (multifunctional DNA repair enzyme) 1 Apex nuclease 1 APEX nuclease APEX1 APEX1_HUMAN Apurinic endonuclease Apurinic-apyrimidinic endonuclease 1 Apurinic/apyrimidinic (abasic) endonuclease Apurinic/apyrimidinic endonuclease 1 Apurinic/apyrimidinic exonuclease APX BAP1 Deoxyribonuclease (apurinic or apyrimidinic) DNA (apurinic or apyrimidinic site) lyase DNA-(apurinic or apyrimidinic site) lyase, mitochondrial EC 4.2.99.18 HAP 1 HAP1 Human Apurinic endonuclease 1 MGC139790 Multifunctional DNA repair enzyme Redox factor 1 Redox factor-1 REF 1 REF 1 protein REF-1 REF1 REF1 protein
Images
ER1802-49_1.jpg Fig1: Western blot analysis of APE1 on different lysates with Rabbit anti-APE1 antibody (ER1802-49) at 1/5,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD APE1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 5 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-49) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1802-49_2.jpg Fig2: Western blot analysis of APE1 on different lysates using anti-APE1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HL-60
Lane 2: Human skin tissue
Lane 3: MCF-7
Lane 4: Mouse placenta tissue
ER1802-49_3.jpg Fig3: ICC staining APE1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-49_4.jpg Fig4: ICC staining APE1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-49_5.jpg Fig5: ICC staining APE1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-49_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-APE1 antibody. Counter stained with hematoxylin.
ER1802-49_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-APE1 antibody. Counter stained with hematoxylin.
ER1802-49_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-APE1 antibody. Counter stained with hematoxylin.
ER1802-49_9.jpg Fig9: Flow cytometric analysis of A549 cells with APE1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.