NEDD8 Rabbit Polyclonal Antibody
cat.: ER1802-5
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 9 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human NEDD8 aa 1-81 / 81.
Positive control: PC-3M, HepG2, MCF-7.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC
  FC

1:500-1:1,000
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: Q15843 Human
Alternative names: FLJ43224 MGC104393 MGC125896 MGC125897 NED8 NEDD 8 NEDD-8 Nedd8 NEDD8_HUMAN Neddylin Neural precursor cell expressed developmentally down regulated 8 Neural precursor cell expressed developmentally down regulated gene 8 Neural precursor cell expressed developmentally down-regulated protein 8 Rub1 Ubiquitin like protein Nedd 8 Ubiquitin like protein Nedd8 Ubiquitin-like protein Nedd8
Images
ER1802-5_1.jpg Fig1: Western blot analysis of NEDD8 on PC-3M cell lysate using anti-NEDD8 antibody at 1/1,000 dilution.
ER1802-5_2.jpg Fig2: ICC staining NEDD8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-5_3.jpg Fig3: ICC staining NEDD8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-5_4.jpg Fig4: ICC staining NEDD8 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-5_5.jpg Fig5: Flow cytometric analysis of MCF-7 cells with NEDD8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.