Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 36 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Annexin A3 aa 2-323. |
Positive control: | Siha, Hela, rat spleen tissue lysate, rat liver tissue lysate, mouse lung tissue lysate, rat kidney tissue, human colon cancer tissue, human spleen tissue, human prostate tissue, A431. |
Subcellular location: | Cytoplasm. Secreted. Plasma Membrane. |
Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P12429 Human | O35639 Mouse | P14669 Rat |
Alternative names: | 2-cyclic phosphate 2-phosphohydrolase 35-alpha calcimedin Annexin A3 Annexin III Annexin-3 ANX3 ANXA 3 Anxa3 ANXA3_HUMAN Calcimedin 35 alpha Inositol 1 2 cyclic phosphate 2 phosphohydrolase Inositol 1 Lipocortin III OTTHUMP00000160701 OTTHUMP00000219237 OTTHUMP00000219238 PAP III PAP-III Placental anticoagulant protein III |
Fig1:
Western blot analysis of Annexin A3 on different lysates using anti-Annexin A3 antibody at 1/1,000 dilution. Positive control: Lane 1: Siha Lane 2: Hela Lane 3: Rat spleen tissue Lane 4: Rat liver tissue Lane 5: Mouse lung tissue |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin. | |
Fig6: Flow cytometric analysis of A431 cells with Annexin A3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |