Pax8 Rabbit Polyclonal Antibody
cat.: ER1802-51
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal Human Pax8.
Positive control: SKOV-3, human thyroid gland tissue, SiHa.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q06710 Human
Alternative names: OTTHUMP00000158659 OTTHUMP00000158660 OTTHUMP00000203723 OTTHUMP00000203724 Paired box 8 Paired box gene 8 paired box homeotic gene 8 Paired box protein Pax 8 Paired box protein Pax-8 Paired domain gene 8 PAX 8 PAX8 PAX8_HUMAN
Images
ER1802-51_1.jpg Fig1: Western blot analysis of Pax8 on human thyroid gland tissue and SKOV-3 cell lysates using anti-Pax8 antibody at 1/500 dilution.
ER1802-51_2.jpg Fig2: ICC staining Pax8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-51_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Pax8 antibody. Counter stained with hematoxylin.
ER1802-51_4.jpg Fig4: Flow cytometric analysis of SiHa cells with Pax8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.