Hip1 Rabbit Polyclonal Antibody
cat.: ER1802-52
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 116 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Hip1 aa 980-1,029 / 1,029. |
| Positive control: | Mouse testis tissue lysate, Mouse spinal cord tissue lysate, A549, PC-3M, SH-SY5Y, human colon cancer tissue, mouse brain tissue, mouse fallopian tubes tissue. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O00291 Human | Q8VD75 Mouse | A0A8I5Y6T0 Rat |
| Alternative names: | 2610109B09Rik A930014B11Rik E130315I21Rik HIP 1 HIP I HIP-1 HIP-I hip1 HIP1/PDGFRB fusion gene HIP1/PDGFRB fusion gene, included HIP1_HUMAN HIPI Huntingtin interacting protein 1 Huntingtin-interacting protein 1 Huntingtin-interacting protein I ILWEQ KIAA4113 MGC126506 MGC27616 mKIAA4113 |
Images
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Fig1:
Western blot analysis of Hip1 on different tissue lysates using anti-Hip1 antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis Lane 2: Mouse spinal cord Lane 3: SH-SY5Y |
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Fig2: ICC staining Hip1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Hip1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Hip1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hip1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hip1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Hip1 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of SH-SY5Y cells with Hip1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.