RAB7 Rabbit Polyclonal Antibody
cat.: ER1802-53
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RAB7 aa 43-200 / 207. |
| Positive control: | MCF-7, rat brain tissue, human brain tissue, human tonsil tissue, human liver tissue, human colon cancer tissue, mouse colon tissue. |
| Subcellular location: | Endosome. Lysosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P51149 Human | P51150 Mouse | P09527 Rat |
| Alternative names: | CMT2B PRO2706 PSN RAB7, member RAS oncogene family RAB7A RAB7A, member RAS oncogene family RAB7A_HUMAN Ras associated protein RAB7 Ras related protein Rab7 Ras related protein Rab7a Ras-related protein Rab-7a |
Images
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Fig1:
Western blot analysis of RAB7 on different lysates with Rabbit anti-RAB7 antibody (ER1802-53) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (20 µg/Lane) Lane 2: Human brain tissue lysate (20 µg/Lane) Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-53) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RAB7 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RAB7 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RAB7 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RAB7 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RAB7 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of A431 cells with RAB7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.