DIAPH3 Rabbit Polyclonal Antibody
cat.: ER1802-54
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 137 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human DIAPH3 aa 1,059-1,108 / 1,193. |
| Positive control: | HeLa cell lysate, human placenta tissue, human kidney tissue, human prostate cancer tissue, human cervical smooth muscle tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:400 |
| Uniprot #: | SwissProt: Q9NSV4 Human |
| Alternative names: | AN AUNA1 Dia2 diap3 DIAP3_HUMAN DIAPH3 Diaphanous homolog 3 (Drosophila) Diaphanous homolog 3 Diaphanous related formin 3 Diaphanous, Drosophila, homolog of, 3 Diaphanous-related formin-3 DKFZp434C0931 DKFZp686A13178 DRF3 FLJ34705 mDia2 NSDAN OTTHUMP00000018480 Protein diaphanous homolog 3 RP11-26P21.1 |
Images
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Fig1:
Western blot analysis of DIAPH3 on different lysates with Rabbit anti-DIAPH3 antibody (ER1802-54) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 137 kDa Observed band size: 137 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-54) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-DIAPH3 antibody (ER1802-54) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-54) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-DIAPH3 antibody (ER1802-54) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-54) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-DIAPH3 antibody (ER1802-54) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-54) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human cervical smooth muscle tissue with Rabbit anti-DIAPH3 antibody (ER1802-54) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-54) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.