DIAPH3 Rabbit Polyclonal Antibody
cat.: ER1802-54
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 137/80 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DIAPH3 aa 1,059-1,108 / 1,193.
Positive control: A431, LOVO, SiHa, human kidney tissue, human placenta tissue.
Subcellular location: Cytosol.
Recommended Dilutions:
  ICC
  IHC-P
  FC
  WB

1:50-1:200
1:50-1:200
1:50-1:100
1:500
Uniprot #: SwissProt: Q9NSV4 Human
Alternative names: AN AUNA1 Dia2 diap3 DIAP3_HUMAN DIAPH3 Diaphanous homolog 3 (Drosophila) Diaphanous homolog 3 Diaphanous related formin 3 Diaphanous, Drosophila, homolog of, 3 Diaphanous-related formin-3 DKFZp434C0931 DKFZp686A13178 DRF3 FLJ34705 mDia2 NSDAN OTTHUMP00000018480 Protein diaphanous homolog 3 RP11-26P21.1
Images
ER1802-54_1.jpg Fig1: Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
ER1802-54_2.jpg Fig2: ICC staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-54_3.jpg Fig3: ICC staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-54_4.jpg Fig4: ICC staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-54_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.
ER1802-54_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.
ER1802-54_7.jpg Fig7: Flow cytometric analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.