Anti-Raptor antibody
cat.: ER1802-57
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 131 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Raptor aa 90-170.
Positive control: MCF-7, 293T, LOVO, rat brain tissue, human breast tissue, mouse colon tissue, A549.
Subcellular location: Cytoplasm. Lysosome.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q8N122 Human | Q8K4Q0 Mouse
Alternative names: KIAA1303 KOG1 Mip1 P150 target of rapamycin (TOR) scaffold protein p150 target of rapamycin (TOR) scaffold protein containing WD repeats P150 target of rapamycin (TOR)-scaffold protein Raptor Regulatory associated protein of mTOR Regulatory associated protein of MTOR complex 1 Regulatory-associated protein of mTOR RPTOR RPTOR_HUMAN
Images
ER1802-57_1.jpg Fig1: Western blot analysis of Raptor on MCF-7 cell lysate using anti-Raptor antibody at 1/500 dilution.
ER1802-57_2.jpg Fig2: ICC staining Raptor in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-57_3.jpg Fig3: ICC staining Raptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-57_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Raptor antibody. Counter stained with hematoxylin.
ER1802-57_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Raptor antibody. Counter stained with hematoxylin.
ER1802-57_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Raptor antibody. Counter stained with hematoxylin.
ER1802-57_7.jpg Fig7: Flow cytometric analysis of A549 cells with Raptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.