Raptor Rabbit Polyclonal Antibody
cat.: ER1802-57
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 149 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Raptor aa 90-170. |
| Positive control: | Mouse brain tissue lysate, 293T, LOVO, rat brain tissue, human breast tissue, mouse colon tissue, A549. |
| Subcellular location: | Cytoplasm, Lysosome, Cytoplasmic granule. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q8N122 Human | Q8K4Q0 Mouse Entrez Gene: 287871 Rat |
| Alternative names: | KIAA1303 KOG1 Mip1 P150 target of rapamycin (TOR) scaffold protein p150 target of rapamycin (TOR) scaffold protein containing WD repeats P150 target of rapamycin (TOR)-scaffold protein Raptor Regulatory associated protein of mTOR Regulatory associated protein of MTOR complex 1 Regulatory-associated protein of mTOR RPTOR RPTOR_HUMAN |
Images
|
Fig1:
Western blot analysis of Raptor on different lysates with Rabbit anti-Raptor antibody (ER1802-57) at 1/500 dilution. Lane 1: 293T (Human embryonic kidney cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-57, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
|
Fig2:
Western blot analysis of Raptor on Mouse brain tissue lysate with Rabbit anti-Raptor antibody (ER1802-57) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-57, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 149 kDa Observed band size: 149 kDa |
|
Fig3: ICC staining Raptor in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining Raptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Raptor antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Raptor antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Raptor antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.