Tle6 Rabbit Polyclonal Antibody
cat.: ER1802-58
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Tle6 aa 157-206 / 581. |
| Positive control: | Mouse ovary tissue lysate, HepG2, A431, A549, SK-Br-3, mouse small intestine tissue, Daudi. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9H808 Human | Q9WVB3 Mouse |
| Alternative names: | Transducin-like enhancer protein 6 TLE6 |
Images
|
Fig1: ICC staining Tle6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig2: ICC staining Tle6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Tle6 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Tle6 antibody. Counter stained with hematoxylin. |
|
Fig5:
Western blot analysis of Tle6 on different lysates with Rabbit anti-Tle6 antibody (ER1802-58) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 62 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-58, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 75 kDa Observed band size: 75 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.