NGF Rabbit Polyclonal Antibody
cat.: ER1802-59
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NGF aa 119-158 / 241. |
| Positive control: | N2A, SHG-44, SH-SY-5Y, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Secreted, Endosome lumen. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P01138 Human | P01139 Mouse | P25427 Rat |
| Alternative names: | Beta nerve growth factor Beta NGF Beta-nerve growth factor Beta-NGF HSAN5 MGC161426 MGC161428 Nerve growth factor (beta polypeptide) Nerve growth factor Nerve growth factor beta Nerve growth factor beta polypeptide Nerve growth factor beta subunit NGF NGF_HUMAN NGFB NID67 |
Images
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Fig1: Western blot analysis of NGF on recombinant protein lysate using anti-NGF antibody at 1/500 dilution. |
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Fig2: ICC staining NGF in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining NGF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining NGF in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NGF antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NGF antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.