CXCL12/SDF1 Rabbit Polyclonal Antibody
cat.: ER1802-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CXCL12 aa 22-93. |
| Positive control: | Rat brain tissue, mouse small intestine tissue, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P48061 Human |
| Alternative names: | 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 AI174028 C-X-C motif chemokine 12 Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) Chemokine (C-X-C motif) ligand 12 Chemokine CXC motif ligand 12 cxcl12 hIRH hSDF-1 Intercrine reduced in hepatomas IRH OTTHUMP00000019491 PBSF Pre-B cell growth-stimulating factor SCYB12 SDF 1 SDF-1 SDF-1-alpha(3-67) SDF-1a SDF-1b SDF1_HUMAN SDF1A SDF1B Stromal cell-derived factor 1 Stromal cell-derived factor 1 delta Stromal cell-derived factor 1 gamma Stromal cell-derived factor 1a Stromal cell-derived factor-1 alpha Thymic lymphoma cell-stimulating factor Tlsf TLSF-a TLSF-b Tlsfa Tlsfb TPAR1 |
Images
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Fig1:
Western blot analysis of CXCL12/SDF1 on CXCL12 recombinant protein with Rabbit anti-CXCL12/SDF1 antibody (ER1802-6) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SDF1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SDF1 antibody. Counter stained with hematoxylin. |
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Fig4: Flow cytometric analysis of HepG2 cells with SDF1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.