ER1802-61

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 41 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within Human Rae1 aa 319-368 / 368.

Positive control:

PC-3, mouse testis tissue, rat testis tissue, LOVO, MCF-7, PC-3M.

Subcellular location:

Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, spindle pole, Nucleus envelope.

Recommended Dilutions:
  WB
  IF-Cell
  FC

1:500-1,000
1:50-1:200
1:50-1:200

Uniprot #:

SwissProt: P78406 Human | Q8C570 Mouse | Q3SWS8 Rat

Alternative names:

dJ481F12.3 dJ800J21.1 FLJ30608 Homolog of yeast Rae1 (Bharathi) mRNA associated protein of 41 kDa (Kraemer) Homolog of yeast Rae1 mRNA associated protein of 41 kDa MGC117333 MGC126076 MGC126077 MIG 14 MIG14 Migration inducing gene 14 Mnrp 41 Mnrp41 mRNA associated protein mrnp 41 mRNA binding protein 41 kD mRNA export factor mRNA export protein mRNA-associated protein mrnp 41 MRNP 41 MRNP41 RAE 1 RAE1 (RNA export 1 S.pombe) homolog rae1 RAE1 homolog Rae1 protein homolog RAE1 RNA export 1 homolog (S. pombe) RAE1 RNA export 1 homolog RAE1L_HUMAN RNA export 1 RNA export 1 homolog

	Fig1: Western blot analysis of Rae1 on Mouse testis tissue lysates with Rabbit anti-Rae1 antibody (ER1802-61) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 1 minutes 50 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-61, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 41 kDa Observed band size: 41 kDa
	Fig2: ICC staining Rae1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining Rae1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: ICC staining Rae1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.