Rae1 Rabbit Polyclonal Antibody
cat.: ER1802-61
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Rae1 aa 319-368 / 368. |
| Positive control: | PC-3, mouse testis tissue, rat testis tissue, LOVO, MCF-7, PC-3M. |
| Subcellular location: | Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, spindle pole, Nucleus envelope. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P78406 Human | Q8C570 Mouse | Q3SWS8 Rat |
| Alternative names: | dJ481F12.3 dJ800J21.1 FLJ30608 Homolog of yeast Rae1 (Bharathi) mRNA associated protein of 41 kDa (Kraemer) Homolog of yeast Rae1 mRNA associated protein of 41 kDa MGC117333 MGC126076 MGC126077 MIG 14 MIG14 Migration inducing gene 14 Mnrp 41 Mnrp41 mRNA associated protein mrnp 41 mRNA binding protein 41 kD mRNA export factor mRNA export protein mRNA-associated protein mrnp 41 MRNP 41 MRNP41 RAE 1 RAE1 (RNA export 1 S.pombe) homolog rae1 RAE1 homolog Rae1 protein homolog RAE1 RNA export 1 homolog (S. pombe) RAE1 RNA export 1 homolog RAE1L_HUMAN RNA export 1 RNA export 1 homolog |
Images
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Fig1:
Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution. Positive control: Lane 1: PC-3 Lane 2: Mouse testis tissue Lane 3: Rat testis tissue |
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Fig2: ICC staining Rae1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Rae1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Rae1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.