GST3 Rabbit Polyclonal Antibody
cat.: ER1802-63
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal human GST3. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, A549, HUVEC, LOVO, human liver cancer tissue, human placenta tissue. |
| Subcellular location: | Cytoplasm. Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:10,000 1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P09211 Human | P19157 Mouse | P04906 Rat |
| Alternative names: | Deafness Deafness X-linked 7 DFN7 FAEES3 Fatty Acid Ethyl Ester Synthase III Glutathione S Transferase 3 Glutathione S Transferase Pi Glutathione S-transferase P Glutathione S-transferase pi 1 GST class-pi GST3 GSTP Gstp1 GSTP1-1 GSTP1_HUMAN PI X linked 7 |
Images
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Fig1:
Western blot analysis of GST3 on different lysates with Rabbit anti-GST3 antibody (ER1802-63) at 1/10,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 5 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-63, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 23 kDa Observed band size: 25 kDa |
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Fig2: ICC staining GST3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining GST3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining GST3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-GST3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GST3 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.