T-bet / Tbx21 Rabbit Polyclonal Antibody
cat.: ER1802-65
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Tbx21 aa 486-535 / 535. |
| Positive control: | NK-92 cell lysates, Raji, human lymph nodes tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC IHC-P |
1:500 1ug/mL 1:500 |
| Uniprot #: | SwissProt: Q9UL17 Human |
| Alternative names: | T bet T box 21 T box expressed in T cells T box protein 21 T box transcription factor TBX21 T cell specific T box transcription factor T cell specific T box transcription factor T bet T PET T-box protein 21 T-box transcription factor TBX21 T-cell-specific T-box transcription factor T-bet TBET TBLYM TBX 21 Tbx21 TBX21_HUMAN TPET Transcription factor TBLYM |
Images
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Fig1:
Western blot analysis of T-bet / Tbx21 on NK-92 cell lysates with Rabbit anti-T-bet / Tbx21 antibody (ER1802-65) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-65) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of Raji cells labeling T-bet / Tbx21. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1802-65, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-T-bet / Tbx21 antibody (ER1802-65) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-65) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.