BNIP1 Rabbit Polyclonal Antibody
cat.: ER1802-66
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BN1P1 aa 27-228 / 228. |
| Positive control: | Rat liver tissue lysate, HepG2, LOVO, human liver tissue, human placenta tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Mitochondrion membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:100 1:50-1:200 1:50-1:500 |
| Uniprot #: | SwissProt: Q12981 Human | Q8VHI8 Rat |
| Alternative names: | BCL2 adenovirus E1B 19kD interacting protein 1 BCL2/adenovirus E1B 19 kDa protein-interacting protein 1 BNIP1 MGC41600 Nip1 OTTHUMP00000161077 OTTHUMP00000161078 OTTHUMP00000161079 OTTHUMP00000223580 sec20 SEC20_HUMAN SEC20L Transformation-related gene 8 protein TRG-8 TRG8 Vesicle transport protein SEC20 |
Images
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Fig1: Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution. |
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Fig2: ICC staining BNIP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining BNIP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-BNIP1 antibody (ER1802-66) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-66) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-BNIP1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.