BNIP1 Rabbit Polyclonal Antibody
cat.: ER1802-66
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 26 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human BN1P1 aa 27-228 / 228.
Positive control: Rat liver tissue lysate, HepG2, LOVO, human liver tissue, human placenta tissue.
Subcellular location: Endoplasmic reticulum membrane, Mitochondrion membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:100
1:50-1:200
1:50-1:500
Uniprot #: SwissProt: Q12981 Human | Q8VHI8 Rat
Alternative names: BCL2 adenovirus E1B 19kD interacting protein 1 BCL2/adenovirus E1B 19 kDa protein-interacting protein 1 BNIP1 MGC41600 Nip1 OTTHUMP00000161077 OTTHUMP00000161078 OTTHUMP00000161079 OTTHUMP00000223580 sec20 SEC20_HUMAN SEC20L Transformation-related gene 8 protein TRG-8 TRG8 Vesicle transport protein SEC20
Images
ER1802-66_1.jpg Fig1: Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.
ER1802-66_2.jpg Fig2: ICC staining BNIP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-66_3.jpg Fig3: ICC staining BNIP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-66_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-BNIP1 antibody (ER1802-66) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-66) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1802-66_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-BNIP1 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.