PARP1 Rabbit Polyclonal Antibody
cat.: ER1802-67
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 113 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PARP1 aa 190-270. |
| Positive control: | HeLa cell lysate, HeLa treated with 1μM staurosporine for 3 hours cell lysate, PC-12 cell lysate, HeLa. |
| Subcellular location: | Nucleus, nucleolus, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: P09874 Human | P27008 Rat |
| Alternative names: | ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) ADP ribosyltransferase ADP ribosyltransferase diphtheria toxin like 1 ADP ribosyltransferase NAD(+) ADPRT 1 ADPRT ADPRT1 ARTD1 msPARP NAD(+) ADP ribosyltransferase 1 NAD(+) ADP-ribosyltransferase 1 pADPRT 1 pADPRT1 PARP 1 PARP PARP-1 PARP1 PARP1_HUMAN Poly (ADP ribose) polymerase 1 poly (ADP ribose) polymerase family, member 1 Poly [ADP-ribose] polymerase 1 Poly(ADP ribose) polymerase poly(ADP ribose) synthetase poly(ADP ribosyl)transferase Poly[ADP ribose] synthetase 1 Poly[ADP-ribose] synthase 1 PPOL |
Images
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Fig1:
Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody (ER1802-67) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 1μM staurosporine for 3 hours cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 113 kDa Observed band size: 113/89 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-67) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling PARP1 with Rabbit anti-PARP1 antibody (ER1802-67) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PARP1 antibody (ER1802-67) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling PARP1. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1802-67, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.