ER1802-67

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 113 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within PARP1 aa 190-270.

Positive control:

Daudi, 293, A431, SH-SY5Y, K562.

Subcellular location:

Nucleus, nucleolus, Cytoplasm, cytosol.

Recommended Dilutions:
  WB
  IF-Cell
  FC

1:500
1:50-1:100
1:50-1:100

Uniprot #:

SwissProt: P09874 Human

Alternative names:

ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) ADP ribosyltransferase ADP ribosyltransferase diphtheria toxin like 1 ADP ribosyltransferase NAD(+) ADPRT 1 ADPRT ADPRT1 ARTD1 msPARP NAD(+) ADP ribosyltransferase 1 NAD(+) ADP-ribosyltransferase 1 pADPRT 1 pADPRT1 PARP 1 PARP PARP-1 PARP1 PARP1_HUMAN Poly (ADP ribose) polymerase 1 poly (ADP ribose) polymerase family, member 1 Poly [ADP-ribose] polymerase 1 Poly(ADP ribose) polymerase poly(ADP ribose) synthetase poly(ADP ribosyl)transferase Poly[ADP ribose] synthetase 1 Poly[ADP-ribose] synthase 1 PPOL

	Fig1: Western blot analysis of PARP1 on Daudi and 293 cell lysates using anti- PARP1 antibody at 1/500 dilution.
	Fig2: ICC staining PARP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining PARP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Flow cytometric analysis of K562 cells with PARP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.