SUFU Rabbit Polyclonal Antibody
cat.: ER1802-68
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SUFU aa 33-260 / 484.
Positive control: 293, SH-SY-5Y, A549, human kidney tissue, human colon cancer tissue, SKOV-3.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1ug/mL
Uniprot #: SwissProt: Q9UMX1 Human
Alternative names: OTTHUMP00000020374 OTTHUMP00000020377 OTTHUMP00000020379 PRO1280 SU FU SU(F)U Su(fu) SUFU SUFU negative regulator of hedgehog signaling SUFU_HUMAN SUFUH SUFUXL Suppressor of fused homolog (Drosophila) Suppressor of fused homolog
Images
ER1802-68_1.jpg Fig1: Western blot analysis of SUFU on 293 cell lysate using anti-SUFU antibody at 1/500 dilution.
ER1802-68_2.jpg Fig2: ICC staining SUFU in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-68_3.jpg Fig3: ICC staining SUFU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-68_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SUFU antibody. Counter stained with hematoxylin.
ER1802-68_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SUFU antibody. Counter stained with hematoxylin.
ER1802-68_6.jpg Fig6: Flow cytometric analysis of SKOV-3 cells labeling SUFU.

Cells were fixed and permeabilized. Then stained with the primary antibody (ER1802-68, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.