CTGF Rabbit Polyclonal Antibody
cat.: ER1802-69
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CTGF aa 147-293 / 349. |
| Positive control: | Mouse smooth muscle tissue lysate, HepG2, HUVEC, SiHa, human endometrium tissue, mouse smooth muscle tissue, rat smooth muscle tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P29279 Human | P29268 Mouse |
| Alternative names: | CCN 2 CCN family member 2 CCN2 Connective tissue growth factor Ctgf CTGF_HUMAN Hcs 24 Hcs24 Hypertrophic chondrocyte specific protein 24 Hypertrophic chondrocyte-specific gene product 24 Hypertrophic chondrocyte-specific protein 24 IBP-8 IGF-binding protein 8 IGFBP-8 IGFBP8 Insulin like Growth Factor Binding Protein 8 Insulin-like growth factor-binding protein 8 MGC102839 NOV 2 NOV2 |
Images
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Fig1: Western blot analysis of CTGF on mouse smooth muscle tissue lysate using anti-CTGF antibody at 1/500 dilution. |
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Fig2: ICC staining CTGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CTGF in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CTGF in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CTGF antibody (ER1802-69) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-69) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue with Rabbit anti-CTGF antibody (ER1802-69) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-69) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue with Rabbit anti-CTGF antibody (ER1802-69) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-69) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.