ER1802-69

CTGF Rabbit Polyclonal Antibody

cat.: ER1802-69

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 38 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human CTGF aa 1-349.
Positive control:	PANC-1 cell lysate, HepG2 cell lysate, HFF-1 cell lysate, Mouse lung tissue lysate, Mouse heart tissue lysate, Rat heart tissue lysate, HepG2, human heart tissue, mouse heart tissue, mouse kidney tissue, rat heart tissue, rat kidney tissue.
Subcellular location:	Secreted, extracellular space, extracellular matrix
Recommended Dilutions: WB IF-Cell IHC-P	1:5,000 1:500 1:200-1:1,000
Uniprot #:	SwissProt: P29279 Human \| P29268 Mouse \| Q9R1E9 Rat
Alternative names:	CCN 2 CCN family member 2 CCN2 Connective tissue growth factor Ctgf CTGF_HUMAN Hcs 24 Hcs24 Hypertrophic chondrocyte specific protein 24 Hypertrophic chondrocyte-specific gene product 24 Hypertrophic chondrocyte-specific protein 24 IBP-8 IGF-binding protein 8 IGFBP-8 IGFBP8 Insulin like Growth Factor Binding Protein 8 Insulin-like growth factor-binding protein 8 MGC102839 NOV 2 NOV2

Images

Fig1: Western blot analysis of CTGF on different lysates with Rabbit anti-CTGF antibody (ER1802-69) at 1/5,000 dilution.

Lane 1: PANC-1 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (negative) (20 µg/Lane)
Lane 4: HFF-1 cell lysate (20 µg/Lane)
Lane 5: Mouse lung tissue lysate (40 µg/Lane)
Lane 6: Mouse heart tissue lysate (40 µg/Lane)
Lane 7: Rat heart tissue lysate (40 µg/Lane)

Predicted band size: 38 kDa
Observed band size: 35 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-69) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunocytochemistry analysis of HepG2 (positive) and MCF7 (low expression) labeling CTGF with Rabbit anti-CTGF antibody (ER1802-69) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CTGF antibody (ER1802-69) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

	Fig3: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-CTGF antibody (ER1802-69) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1802-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-CTGF antibody (ER1802-69) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1802-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CTGF antibody (ER1802-69) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1802-69) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-CTGF antibody (ER1802-69) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1802-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CTGF antibody (ER1802-69) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1802-69) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.