PKM2 Rabbit Polyclonal Antibody
cat.: ER1802-70
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 58 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PKM2 aa 370-450.
Positive control: Wild-type MDA-MB-231 whole cell lysate, SiHa cell lysate, A549 cell lysate, PC-3 cell lysate, A549, F9, PC-3M, human tonsil tissue, human thyroid carcinoma tissue, human colon cancer tissue, human breast cancer tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:1,000
1:100-1:500
1:50-1:400
Uniprot #: SwissProt: P14618 Human
Alternative names: CTHBP Cytosolic thyroid hormone binding protein Cytosolic thyroid hormone-binding protein KPYM_HUMAN MGC3932 OIP 3 OIP-3 OIP3 OPA interacting protein 3 Opa-interacting protein 3 p58 PK muscle type PK, muscle type PK2 PK3 PKM PKM2 pykm Pyruvate kinase 2/3 Pyruvate kinase 3 Pyruvate kinase isozymes M1/M2 Pyruvate kinase muscle Pyruvate kinase muscle isozyme pyruvate kinase PKM Pyruvate kinase, muscle 2 TCB THBP1 Thyroid hormone binding protein 1 Thyroid hormone binding protein cytosolic Thyroid hormone-binding protein 1 Tumor M2 PK Tumor M2-PK
Images
ER1802-70_1.jpg Fig1: All lanes: Western blot analysis of PKM with anti-PKM antibody (ER1802-70) at 1:500 dilution.

Lane 1: Wild-type MDA-MB-231 whole cell lysate.
Lane 2: PKM knockout MDA-MB-231 whole cell lysate.

ER1802-70 was shown to specifically react with PKM in wild-type MDA-MB-231 cells. No band was observed when PKM knockout samples were tested. Wild-type and PKM knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PKM antibody (ER1802-70, 1/500) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
ER1802-70_2.jpg Fig2: Western blot analysis of PKM2 on different lysates with Rabbit anti-PKM2 antibody (ER1802-70) at 1/500 dilution.

Lane 1: SiHa cell lysate
Lane 2: A549 cell lysate
Lane 3: PC-3 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 58 kDa
Observed band size: 58 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-70) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1802-70_3.jpg Fig3: ICC staining PKM2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-70_4.jpg Fig4: ICC staining PKM2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-70_5.jpg Fig5: ICC staining PKM2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-70_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
ER1802-70_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-PKM2 antibody (ER1802-70) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-70) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1802-70_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
ER1802-70_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.