Heme Oxygenase 1 (HO-1) Rabbit Polyclonal Antibody
cat.: ER1802-73
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide of N-terminal human Heme Oxygenase 1 (HO-1). |
| Positive control: | Mouse spleen tissue lysate, 293T, HepG2, human spleen tissue, human placenta tissue, human small intestine tissue, mouse small intestine tissue, A549-si NT cell lysate. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P09601 Human | P14901 Mouse |
| Alternative names: | 32 kD bK286B10 D8Wsu38e heat shock protein 32 kD heat shock protein 32kD Heat shock protein Heme oxygenase (decycling) 1 Heme oxygenase 1 Hemox HMOX 1 Hmox Hmox1 HMOX1_HUMAN HO 1 HO HO-1 HO1 Hsp32 |
Images
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Fig1: Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution. |
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Fig2:
Western blot analysis of HO-1 on different lysates with Rabbit anti-HO-1 antibody (ER1802-73) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si HO-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. ER1802-73 was shown to specifically react with HO-1 in A549-si NT cells. Weakened band was observed when A549-si HO-1 sample was tested. A549-si NT and A549-si HO-1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1802-73, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) were used in 5% BSA at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining Heme Oxygenase 1 (HO-1) in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Heme Oxygenase 1 (HO-1) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.