Securin Rabbit Polyclonal Antibody
cat.: ER1802-75
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Securin aa 2-202. |
| Positive control: | HUVEC cell lysate, Daudi cell lysate, HeLa cell lysate, HCT 116 cell lysate, HepG2 cell lysate, HUVEC, PC-3M, human tonsil tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:500 1:200-1:500 1:200-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: O95997 Human |
| Alternative names: | AW555095 C87862 Cut2 EAP 1 EAP1 ESP1 associated protein 1 Esp1-associated protein hPTTG MGC126883 MGC138276 Pds1 Pituitary tumor transforming 1 Pituitary tumor transforming protein 1 Pituitary tumor-transforming 1, isoform CRA_a Pituitary tumor-transforming 1, isoform CRA_b Pituitary tumor-transforming gene 1 Pituitary tumor-transforming gene 1 protein PTTG 1 PTTG PTTG1 PTTG1 protein PTTG1_HUMAN Pttg3 Securin Tumor transforming 1 Tumor transforming protein 1 Tumor-transforming protein 1 TUTR 1 TUTR1 |
Images
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Fig1:
Western blot analysis of Securin on different lysates with Rabbit anti-Securin antibody (ER1802-75) at 1/500 dilution. Lane 1: HUVEC cell lysate Lane 2: Daudi cell lysate Lane 3: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-75, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22 kDa Observed band size: 25 kDa, 28 kDa |
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Fig2:
Western blot analysis of Securin on different lysates with Rabbit anti-Securin antibody (ER1802-75) at 1/500 dilution. Lane 1: HCT 116 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-75, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22 kDa Observed band size: 28 kDa |
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Fig3: ICC staining Securin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Securin antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.