Securin Rabbit Polyclonal Antibody
cat.: ER1802-75
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 22 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Securin aa 2-202.
Positive control: HUVEC, PC-3M, human tonsil tissue, mouse testis tissue, rat testis tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  ICC
  FC

1:500
1:200-1:500
1:200-1:500
1:50-1:100
Uniprot #: SwissProt: O95997 Human
Alternative names: AW555095 C87862 Cut2 EAP 1 EAP1 ESP1 associated protein 1 Esp1-associated protein hPTTG MGC126883 MGC138276 Pds1 Pituitary tumor transforming 1 Pituitary tumor transforming protein 1 Pituitary tumor-transforming 1, isoform CRA_a Pituitary tumor-transforming 1, isoform CRA_b Pituitary tumor-transforming gene 1 Pituitary tumor-transforming gene 1 protein PTTG 1 PTTG PTTG1 PTTG1 protein PTTG1_HUMAN Pttg3 Securin Tumor transforming 1 Tumor transforming protein 1 Tumor-transforming protein 1 TUTR 1 TUTR1
Images
ER1802-75_1.jpg Fig1: Western blot analysis of Securin on HUVEC cell lysate using anti-Securin antibody at 1/500 dilution.
ER1802-75_2.jpg Fig2: ICC staining Securin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-75_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Securin antibody. Counter stained with hematoxylin.
ER1802-75_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse tetis tissue using anti-Securin antibody. Counter stained with hematoxylin.
ER1802-75_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Securin antibody. Counter stained with hematoxylin.
ER1802-75_6.jpg Fig6: Flow cytometric analysis of PC-3M cells with Securin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.