BubR1 Rabbit Polyclonal Antibody
cat.: ER1802-76
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 120 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BubR1 aa 310-620. |
| Positive control: | SH-SY-5Y cell lysate, Hela, SH-SY-5Y, SiHa, human spleen tissue, HL-60. |
| Subcellular location: | Nucleus, Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000-1:10,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O60566 Human |
| Alternative names: | Beta homolg of S. cerevisiae BUB 1 Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles BUB 1B BUB1 budding uninhibited by benzimidazoles 1 homolog beta Bub1A BUB1B BUB1B_HUMAN BUB1beta BUBR1 Budding Uninhibited by Benzimidazoles 1 beta Budding uninhibited by benzimidazoles 1 homolog beta (yeast) hBUBR1 MAD3/BUB1 related protein kinase MAD3/BUB1-related protein kinase MAD3L Mitotic checkpoint gene BUB1B Mitotic checkpoint kinase MAD3L Mitotic checkpoint serine/threonine protein kinase BUB1 beta Mitotic checkpoint serine/threonine-protein kinase BUB1 beta MVA1 OTTHUMP00000160319 Protein SSK1 SSK 1 SSK1 |
Images
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Fig1: Western blot analysis of BubR1 on SH-SY-5Y cell lysate using anti-BubR1 antibody at 1/10,000 dilution. |
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Fig2: ICC staining BubR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining BubR1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining BubR1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BubR1 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of HL-60 cells with BubR1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.