BubR1 Rabbit Polyclonal Antibody
cat.: ER1802-76
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 120 kDa
Isotype: IgG
Immunogen: Recombinant protein within human BubR1 aa 310-620.
Positive control: SH-SY-5Y cell lysate, Hela, SH-SY-5Y, SiHa, human spleen tissue, HL-60.
Subcellular location: Nucleus, Cytoplasm, Cytoskeleton.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:5,000-1:10,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O60566 Human
Alternative names: Beta homolg of S. cerevisiae BUB 1 Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles BUB 1B BUB1 budding uninhibited by benzimidazoles 1 homolog beta Bub1A BUB1B BUB1B_HUMAN BUB1beta BUBR1 Budding Uninhibited by Benzimidazoles 1 beta Budding uninhibited by benzimidazoles 1 homolog beta (yeast) hBUBR1 MAD3/BUB1 related protein kinase MAD3/BUB1-related protein kinase MAD3L Mitotic checkpoint gene BUB1B Mitotic checkpoint kinase MAD3L Mitotic checkpoint serine/threonine protein kinase BUB1 beta Mitotic checkpoint serine/threonine-protein kinase BUB1 beta MVA1 OTTHUMP00000160319 Protein SSK1 SSK 1 SSK1
Images
ER1802-76_1.jpg Fig1: Western blot analysis of BubR1 on SH-SY-5Y cell lysate using anti-BubR1 antibody at 1/10,000 dilution.
ER1802-76_2.jpg Fig2: ICC staining BubR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-76_3.jpg Fig3: ICC staining BubR1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-76_4.jpg Fig4: ICC staining BubR1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-76_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BubR1 antibody. Counter stained with hematoxylin.
ER1802-76_6.jpg Fig6: Flow cytometric analysis of HL-60 cells with BubR1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.