| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide of C-terminal human RUNX2. |
| Positive control: | HL-60 cell lysate, A431, human tonsil tissue, human colon tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q13950 Human |
| Alternative names: | Acute myeloid leukemia 3 protein Alpha subunit 1 AML3 CBF alpha 1 CBF-alpha-1 CBFA1 CCD CCD1 Cleidocranial dysplasia 1 Core binding factor Core binding factor runt domain alpha subunit 1 Core binding factor subunit alpha 1 Core-binding factor subunit alpha-1 MGC120022 MGC120023 Oncogene AML 3 Oncogene AML-3 OSF 2 OSF-2 OSF2 Osteoblast specific transcription factor 2 Osteoblast-specific transcription factor 2 OTTHUMP00000016533 PEA2 alpha A PEA2-alpha A PEA2aA PEBP2 alpha A PEBP2-alpha A PEBP2A1 PEBP2A2 PEBP2aA PEBP2aA1 Polyomavirus enhancer binding protein 2 alpha A subunit Polyomavirus enhancer-binding protein 2 alpha A subunit Runt domain Runt related transcription factor 2 Runt-related transcription factor 2 RUNX2 RUNX2_HUMAN SL3 3 enhancer factor 1 alpha A subunit SL3-3 enhancer factor 1 alpha A subunit SL3/AKV core binding factor alpha A subunit SL3/AKV core-binding factor alpha A subunit |
|
Fig1: Western blot analysis of RUNX2 on HL-60 cell lysate using anti-RUNX2 antibody at 1/500 dilution. |
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Fig2: ICC staining RUNX2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX2 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RUNX2 antibody. Counter stained with hematoxylin. |