ER1802-78

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IHC-P, IF-Cell
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 57 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide of C-terminal human RUNX2.

Positive control:

HL-60 cell lysate, A431, human tonsil tissue, human colon tissue.

Subcellular location:

Nucleus.

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500
1:50-1:200
1:50-1:200

Uniprot #:

SwissProt: Q13950 Human

Alternative names:

Acute myeloid leukemia 3 protein Alpha subunit 1 AML3 CBF alpha 1 CBF-alpha-1 CBFA1 CCD CCD1 Cleidocranial dysplasia 1 Core binding factor Core binding factor runt domain alpha subunit 1 Core binding factor subunit alpha 1 Core-binding factor subunit alpha-1 MGC120022 MGC120023 Oncogene AML 3 Oncogene AML-3 OSF 2 OSF-2 OSF2 Osteoblast specific transcription factor 2 Osteoblast-specific transcription factor 2 OTTHUMP00000016533 PEA2 alpha A PEA2-alpha A PEA2aA PEBP2 alpha A PEBP2-alpha A PEBP2A1 PEBP2A2 PEBP2aA PEBP2aA1 Polyomavirus enhancer binding protein 2 alpha A subunit Polyomavirus enhancer-binding protein 2 alpha A subunit Runt domain Runt related transcription factor 2 Runt-related transcription factor 2 RUNX2 RUNX2_HUMAN SL3 3 enhancer factor 1 alpha A subunit SL3-3 enhancer factor 1 alpha A subunit SL3/AKV core binding factor alpha A subunit SL3/AKV core-binding factor alpha A subunit

	Fig1: Western blot analysis of RUNX2 on HL-60 cell lysate using anti-RUNX2 antibody at 1/500 dilution.
	Fig2: ICC staining RUNX2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.
	Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.